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. 2017 Jul 19;7:5801. doi: 10.1038/s41598-017-06374-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation of apical and basal dendrites and spines of layer-Va neurons in Pcdh19 mutants. (a) Representative layer Va neurons labeled by in utero electroporation of GFP. Arrows indicate the apical dendrite of each neuron. Brains were fixed at P21. (b) Examples of dendritic branches of layer Va neurons. Tracings of dendrites and axons were shown in black and magenta, respectively. ap, apical dendrite. (c) Examples of spines along basal dendrites of layer Va neurons. (d) Number of spines per 10 μm along basal dendrites did not significantly differ among wild-type and Pcdh19 mutant mice (P = 0.6184, n = 5 neurons). Scale bars, 100 μm in (a,b) 10 μm in (c). 100 μm-thick slices were used for analysis.