Table 1.

Phenotypes of Pcdh19 hemizygous male (X⁻Y) and heterozygous female (X⁺X⁻) mutants compared to wild-type counterparts.

Test	Measure	Phenotype
		Male	Female
1. General health/neurological screen (GHNS)	Whisker, coat, reflexes	n.s.	n.s.
	Body weight (g)	Decreased	n.s.
	Rectal temperature (°C)	n.s.	Decreased
	Grip strength (N)	Decreased	n.s.
	Wire hang (sec)	n.s.	Decreased
2. Light/Dark transition	Distance traveled in light (cm)	n.s.	Increased
	Distance traveled in dark (cm)	n.s.	Increased
	Stay time in light (sec)	n.s.	n.s.
	Stay time in dark (sec)	n.s.	n.s.
	Number of transitions	n.s	n.s.
	Latency to enter light (sec)	n.s	n.s.
3. Open field	Total distance (cm)	See Fig. S5	See Fig. S5
	Vertical activity	See Fig. S5	See Fig. S5
	Center time (sec)	See Fig. S5	See Fig. S5
	Stereotypic Counts	See Fig. S5	See Fig. S5
4. Elevated plus maze	Distance traveled (cm)	Decreased	Decreased
	Time on open arms (%)	n.s.	n.s.
	Number of entries	Decreased	n.s.
	Entries into open arms (%)	n.s.	n.s.
5. Hot Plate	Latency (sec)	Decreased	n.s
6. Social Interaction (Novel environment)	Total duration of contacts (sec)	n.s.	n.s.
	Mean duration per contacts (sec)	n.s.	n.s.
	Number of contacts	n.s.	n.s.
	Total duration of active contact (sec)	n.s.	n.s.
	Distance traveled (cm)	n.s.	n.s.
7. Rotarod	Latency to fall (sec)	n.s.	n.s.
8. Social interaction (Crawly’s version)	Total distance (cm)	n.s.	n.s.
	Average speed (cm/sec)	n.s.	n.s.
	Sociability	n.s.	n.s.
	Social preference	n.s.	n.s.
9. Prepulse inhibition	Acoustic startle response at 110 dB	n.s.	Increased
	Acoustic startle response at 120 dB	n.s.	n.s.
	Prepulse inhibition (%) at 110 dB	n.s.	n.s.
	Prepulse inhibition (%) at 120 dB	n.s.	n.s
10. Porsolt forced swim	Immobility (%)	See Fig. 5	See Fig. 5
	Distance traveled (cm)	Decreased	n.s.
11. T-maze spontaneous alternation	Correct responses (%), latency (sec) and distance traveled (cm) during training	See Fig. S6	See Fig. S6
	Correct responses in the delay session	See Fig. S6	See Fig. S6
12. Barnes maze	Distance (cm), latency (sec), search errors and omission errors	See Fig. S7	See Fig. S7
	Probe test 1 day after training	See Fig. S8	See Fig. S8
	Probe test 32 days after training	See Fig. S8	See Fig. S8
13. Tail suspension	Immobility (%)	See Fig. 5	See Fig. 5
	Distance traveled (cm)	Increased	Increased
14. Fear conditioning	Freezing (%) and distance traveled (cm) in	See Fig. 7	See Fig. 7
	2) context test 1 day and 28 days after conditioning.	See Fig. 7	See Fig. 7
	3) cued test with altered context 1 day and 28 days after conditioning.	See Fig. 7	See Fig. 7
15. Open field (2nd)	Total distance (cm)	See Fig. 6	See Fig. 6
	Vertical activity	See Fig. 6	See Fig. 6
	Center time (sec)	See Fig. 6	See Fig. 6
	Stereotypic Counts	See Fig. 6	See Fig. 6
16. Social interaction test in a home cage	Social interaction activity	n.s.	n.s.
	Locomotor activity	n.s.	n.s.

n.s., no statistical difference (P > 0.05); Increased, statistically increased (<0.05); Decreased, statistically decreased (<0.05).