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. 2017 Jul 19;7:5862. doi: 10.1038/s41598-017-06180-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative analysis of FND uptake by Hxt6-GFP-expressing Saccharomyces cerevisiae cells. The amount of objects (adjacent FND positive pixels are counted as an object) and particles (an object can also be an aggregate consisting of more than one particle) is estimated through our home written FIJI protocol (see Method section: FND uptake quantification). For all situations, 2 times approximately 100 cells were selected and cells with obvious large aggregates on the exterior were excluded post-hoc. (A and B) show the absolute numbers for both types of transformations. In (C and D) a grouped distribution of the percentage of cells carrying a range of objects is shown. Significance is tested compared to the control situation. *p < 0.05, ***p < 0.001, ****p < 0.0001.