Fig. 1.

SDS-PAGE profile and fibrinolytic activity of NnV. a We compared the fibrinolytic activity between jellyfish species. Lane 1 – N. nomurai; lane 2 – A. aurita; lane 3 – D. quinquecirrha; lane 4 – P. physalis, lane 5 – C. brevipedalia. b NnV (50 μg) was submitted to SDS electrophoresis under non-reducing conditions. The gels were stained with 0.125% Coomassie blue. c Fibrin zymography of NnV with various protease inhibitors, including PMSF (1 mM), BZ (2 mM), EDTA (2 mM) and PT (2 mM). Clear zones in the fibrin gel indicated regions of proteolytic activity