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. 2017 Jul 19;18:12. doi: 10.1186/s12858-017-0087-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Luciferase assay optimization. a ATP titration: The graph represents the light intensity of each reaction containing different ATP concentrations as indicated. b pH titration: The graph represents the light intensity of each reaction at different pH. c Luciferin titration: The graph represents the light intensity of each reaction using different luciferase concentrations as indicated. Graphs A, B and C are in logarithm scale. d Light emission decay time: The graph represents the time that the red luciferase emits light. The means and standard deviations of all graphs were obtained from quadruplicate experiments