Fig. 5.

Features of NR2F2 and ENSMUST00000189966. a Gene structure of NR2F2 and ENSMUST00000189966. NR2F2–001, NR2F2–002 and NR2F2–003 are different isoforms. The scale in the top show the location in the chromosome 7. ENSMUST00000189966 exon 1 overlapped with NR2F2–002 intron 2. b Q-PCR analysis of NR2F2 and ENSMUST00000189966 gene expression during 3 T3-L1 cell culture in the differentiation medium (DM) for 0, 2, 4, and 8 days. c qPCR analyses of cytosolic (Cyt) and nuclear (Nuc) fractions. d The effect of dihydrotestosterone (DHT) (control (CTL), 10⁻⁹, and 10⁻¹⁰ M) on NR2F2 and ENSMUST00000189966 gene expression in 3 T3-L1 cells. The values represent the means ± s.e.m., n = 3. **indicates P < 0.01, *indicates P < 0.05