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. 2017 Apr 27;232(10):2776–2787. doi: 10.1002/jcp.25943

Figure 5.

Figure 5

The influence of Drp1 on mitochondrial fission and cell apoptosis in MPC5 cells. (a) Drp1 expression in GFP‐Drp1‐ or siRNA‐infected MPC5 cells. (b) Transmission electron microscopeimages of the mitochondria in MPC5 cells (×25,000). (c) Mitochondrial morphology in GFP‐Drp1‐infected MPC5 cells (×2500). (d) Apoptosis of GFP‐Drp1‐infected MPC5 cells measured by Hoechst 33258 staining (×200). (e) The quantitative determination of cellular apoptosis in GFP‐Drp1‐infected MPC5 cells using flow cytometry. (f) Western blot analysis for caspase‐3 (cleaved form), nephrin and podocin expression in GFP‐Drp1‐infected MPC5 cells. (g) Apoptosis of Drp1 siRNA‐infected MPC5 cells measured by Hoechst 33258 staining (×200). (h) The quantitative determination of cellular apoptosis in Drp1 siRNA‐infected MPC5 cells using flow cytometry. (i) Western blot analysis for caspase‐3 (cleaved form), nephrin, and podocin expression in Drp1 siRNA‐infected MPC5 cells. GFP‐NG, MPC5 cells infected with empty lentivirus; GFP‐Drp1, MPC5 cells infected with lentivirus expressing Drp1; Drp1 siRNA, MPC5 cells infected with Drp1 siRNA; Scramble, MPC5 cells infected with lentivirus expressing scramble oligonucleotide; Scramble siRNA, MPC5 cells infected with lentivirus expressing scramble siRNA; HG, MPC5 cells cultured in 30 mM D‐glucose; HG + PD, MPC5 cells cultured in 30 mM glucose and 25 mM PD; NG + PD, MPC5 cells cultured in 5.3 mM glucose and 25 mM PD. The results are presented as the means ± SE. *p < 0.05 versus NG(GFP‐NG); #p < 0.05 versus HG(GFP‐Drp1)