Figure 6.

PD attenuated ROS production in HG‐induced podocyte. (a) Flow cytometry analysis of ROS levels in MPC5 cells. (b) Flow cytometry analysis of ROS levels in primary podocytes from KKAy mice. (c) Western blot analysis for Drp1 levels in MPC5 cells. (d) Western blot analysis for Drp1 levels in primary KKAy podocytes. NG, MPC5 cells (primary podocytes) cultured in 5.3 mM D‐glucose; HG, MPC5 cells (primary podocytes) cultured in 30 mM D‐glucose; HG + PD, MPC5 cells (primary podocytes) cultured in 30 mM D‐glucose and 25 mM PD; NG + PD, MPC5 cells (primary podocytes) cultured in 5.3 mM D‐glucose and 25 mM PD; HG + PD + H2O₂, MPC5 cells (primary podocytes) treated with 30 mM D‐glucose, 25 mM PD and 1 µmol/L H₂O₂; H₂O₂, MPC5 cells (primary podocytes) cultured in 5.3 mM D‐glucose and 1 µmol/L H₂O₂; HG + NAC, MPC5 cells (primary podocytes) cultured in 30 mM D‐glucose and 0.4 mmol/L NAC. The results are presented as the mean ± SE. *p < 0.05 versus NG; #p < 0.05 versus HG