Table 2.
Overview of genomes detected in the metagenome.
| Well Sample | N50 | Taxonomic affiliation based on 16S rRNA and single copy gene assignment | Binned genome size, Mbp (contig size range, bp) [reference genome size]a | Blastn 16S rRNA taxonomic affiliation of against NCBI NR database (full length unless stated) | Blastn comparison of binned 16S rRNA gene to Illumina generated OTU | # Contigs | # cds | Taxonomic distribution of ORF |
Genome Completenessb |
Abundance in Metagenome (%) |
Abundance of Corresponding OTU (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 10 | 36 674 | Sulfurospirillum M102 |
2.6 (1000–24 067) [2.3–3.2] |
Sulfurospirillum deleyianum DSM 6946 (98%) | OTU 6 (100%) | 590 | 2559 |
Campylobacterales (92%); Epsilonproteobacteria (95%) |
100% | 18 | 7 |
| Rhizobium MD101 |
6.8 (1015–292 738) [4.9–7.5] |
Rhizobium petrolearium strain SL‐1 (100%) | OTU 2 (100%) | 164 | 6755 |
Rhizobiales (88%); Alphaproteobacteria (93%) |
100% | 72 | 65 | ||
| 3 | 14 942 | Desulfovibrio MD33 |
5.0 (1001–68 886) [3.2–5.25] |
Desulfovibrio marrakechensis strain EMSSDQ4 (98%) | OTU 47 (99.2%) | 767 | 4545 | Desulfovibrionales (92%) | 100% | 12 | 4 |
| Acetobacterium MD34 |
4.0 (1011–114 001) [4.04–4.05] |
Acetobacterium carbinolicum (100%) | OTU 21 (100%) | 315 | 3847 |
Eubacteriaceae (73%); Clostridiales (86%); Firmicutes (94%) |
100% | 5 | 11 | ||
| Sulfurospirillum M31c |
2.6 N/A [2.3–3.2] |
Sulfurospirillum deleyianum DSM 6946 (97%) Sulfurospirillum sp. M10 (100%); 523 bp fragment |
Binned fragment does not overlap with Illumina OTU; OTU 6e |
34 | 2776 |
Campylobacterales (92%); Epsilonproteobacteria (95%) |
100% | 36 | 32 | ||
| Sulfurospirillum M32 |
3.2 N/A [2.3–3.2] |
Sulfurospirillum sp. JPD‐1 (99%); 676 bp fragment |
Binned fragment does not overlap with Illumina OTU; OTU 626e |
29 | 3095 |
Campylobacterales (88%); Epsilonproteobacteria (92%) |
100% | 27 | 21 | ||
| Oscillibacter MD34 |
3.2 (1013–160 132) [3.1–4.4] |
Oscillibacter valericigenes strain Sjm18‐20 (96%) | OTU 12 (100%) | 130 | 3278 |
Oscillospiraceae (42%); Clostridiales (66%); Firmicutes (88%) |
100% | 17 | 17 | ||
| Desulfosporosinus sp. | Not binned |
Peptococcaceae Desulfosporosinus orientis (OTU affiliation) |
No fragment available; OTU 44a |
N/A | Peptococcaceae (100%) | N/A | 6 | ||||
aReference genome sizes obtained from related genera at the NCBI genome database.
bGenome completeness computed based on comparison to 108 single copy genes (SGC) detected in bacterial genomes.
cMethod for binning two Sulfurospirillum strains in Well 3 discussed in Supporting Information.