Figure 6.

Smad4 specifically binds to the promoter of NBCe1 in mouse primary cortical astrocytes after TGF‐β treatment. (a) Smad4 binding site (red) to the NBCe1 promoter region. Underlined sequences represent primer sequences used to detect Smad4 binding. (b) PCR following chromatin immunoprecipitation assay of control and TGF‐β‐treated mouse primary cortical astrocytes demonstrates increased binding of Smad4 to the predicted binding sequence in the promoter region of NBCe1. Predicted PCR product size 89 bp. Histone 3 (H3), and mouse IgG represent positive and negative control, respectively. The figure is a representative of n= 2. (c) Proposed model for TGF‐β‐dependent NBCe1 regulation