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. 2017 Apr 27;30(8):e2621. doi: 10.1002/jmr.2621

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© 2017 The Authors Journal of Molecular Recognition Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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jmr2621-fig-0002 — Assay setup for SPR‐based interaction studies between immobilized Ng_fl and CaM. A, affinity capture of Ng_fl to SPR biosensor surfaces by covalently immobilized polyhistidine antibody; B, stabilization period; C, injection of CaM under ${Ca}^{2 +}$ ‐saturated (gray) and ${Ca}^{2 +}$ ‐depleted conditions (black); D, surface regeneration