Figure 4.

Kinetic analysis of CaM‐CaMKII_290−309 interactions. A, CaMKII_290−309 was injected over immobilized CaM under ${\text{Ca}}^{2+}$‐saturated conditions (left) and ${\text{Ca}}^{2+}$‐depleted conditions (shown in insert, left). A reversible 1‐step model (Scheme 1) was fitted to the double‐referenced sensorgrams (green solid lines). The affinity was determined by fitting Equation 1 to the steady‐signals extracted from the double‐referenced sensorgrams (right). B, Simulation of the effect of different values of the mass transport coefficient (k _t) on the kinetics of the CaMKII_290−309‐CaM interaction under ${\text{Ca}}^{2+}$‐saturated conditions. C, Effect of varying injection times (blue: 30 s, red: 60 s) on interactions between CaMKII_290−309 and CaM under ${\text{Ca}}^{2+}$‐saturated conditions