Table 1.

Affinities for ${\text{Ca}}^{2+}$ interactions with CaM, studied by SPR, MST, and UV fluorescence spectroscopy. Data is presented as the average K _D from the indicated number of replicate experiments (n) and the corresponding standard error (SE)

Method	K D [M] (n)	T [°C]
SPR	(3.2±0.2)×10−6 (5)	22
SPR	(2.8±0.3)×10−6 (4)	25
MST	(3.0±0.5)×10−6 (4)	25
UV	(3.1±0.2)×10−6* (4)	22

*E C ₅₀ at total CaM concentration 1 μM, with the slope factor h=1.6