Skip to main content
. 2017 May 19;141(3):540–548. doi: 10.1002/ijc.30763

Figure 4.

Figure 4

Effect of HPV and 8q24.22 knock‐out on MYC expression. (a, b) The efficiency of HPV DNA knockout in two HPV‐ko HeLa cell strains detected by southern blot analysis (a) and real‐time PCR (b). (c) Relative expression of MYC in HPV‐ko HeLa cell strains detected by Real‐time PCR. (d) Relative expression of MYC in 8q24.22 double (#1) or single (#2–9) knock‐out HeLa cell strains detected by Real‐time PCR. (e) The heterozygous SNP (rs4645948, T/C) in MYC DNA and cDNA were detected by PCR and sequencing in wild‐type, HPV‐ko and 8q24.22‐ko HeLa cells. [Color figure can be viewed at wileyonlinelibrary.com]