FIG 5 .

PI3K modulates TepP-dependent type I IFN responses. HeLa-Cas and PI3KCA KO cells were infected with CTL2 or ΔtepP mutants, and IFIT1 (black bars) or IFIT2 (gray bars) expression was assessed at 16 hpi by quantitative RT-PCR. Results from WT and DNA samples for both IFIT1 and IFIT2 are significantly different between HeLa-Cas and PI3KCA knockout cells (KO) (P value = <0.001, log-transformed ANOVA with a priori contrasts). Fold change values were calculated compared to mock-infected isolates. DNA transfections were included as controls for the induction of IFIT genes. The difference between HeLa-Cas and PI3KCA KO cell responses to DNA transfection or CTL2 infection was statistically significant (P value = <0.01 [Student’s t test]).