Table 1. Luminescence Decay Times of Four Ru–P450 Conjugates in the Absence and Presence of Quenchersa.
| enzyme | quencher | τmono, ns | τa, ns (ρa) | τb, ns (ρb) |
|---|---|---|---|---|
| RuC97(CYP102A1)W96 | none | 140 | 190 (0.65) | 52 (0.35) |
| [Ru(NH3)6]3+ | 30 | |||
| pMeODMA | 62 | |||
| RuC97(CYP102A1)W96H | none | 180 | 160 (0.80) | 310 (0.20) |
| [Ru(NH3)6]3+ | 33 | |||
| pMeODMA | 65 | |||
| RuC77(CYP119)H76 | none | 200 | 220 (0.85) | 45 (0.15) |
| [Ru(NH3)6]3+ | 91 | |||
| pMeODMA | 54 | |||
| RuC77(CYP119)H76W | none | 130 | 91 (0.75) | 320 (0.25) |
| [Ru(NH3)6]3+ | 48 | |||
| pMeODMA | 50 |
a
Quenchers: [Ru(NH3)6]3+, 17 mM; pMeODMA, 10 mM. The relative amplitudes of major (ρa) and minor (ρb) components in biexponential fits to the unquenched decays also are listed. Samples were excited at 480 nm, and luminescence was detected at 630 nm. Uncertainties in the decay times are ±10%, except for the single-exponential fits to the unquenched decays.