Figure 3.

Functional characterization of hPP compounds fatty acid acylated at a) position 22 and b) residue 30 at COS-7 cells stably expressing the anorexigenic hY₄R and hY₂R as well as the orexigenic hY₁R and hY₅R. Co-expression of hYR and a chimeric G_i/q protein allowed concentration-dependent radioactive inositol phosphate accumulation. Mean concentration–response curves of at least two independent experiments, fitted by non-linear regression (GraphPad Prism 5.0) are shown with ±SEM. Dashed black lines correspond to the respective native ligands (hPP for hY₄R and porcine NPY (pNPY) for hY₁R, hY₂R and hY₅R).