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SPR sensorgrams of binding between mono-, bi- and trivalent glycopeptides (5, 7 and 9) and antibody Fab fragments. a–c: with PGT128 Fab; d-f: with 10-1074 Fab. The Fab fragments were run from 1000 nM with 1:2 serial dilutions. Data were fit with a 1:1 Langmuir binding model (fitting was shown in black). Ka was given in M−1S−1; Kd in S−1 and KD in M.
