Figure 3.

SPR sensorgrams of the binding between antibody Fab fragments and nonglycosylated trivalent V3 peptide or trivalent glycans. a) with trivalent V3 peptide (10); b) with trivalent Man₉GlcNAc₂ glycan (11). Fabs were run from 1000 nM with 1:2 serial dilutions. Data were fit with a 1:1 Langmuir binding model (fitting was shown in black). K_a was given in M⁻¹S⁻¹; K_d in S⁻¹ and K_D in M.S1)