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Formation of MCF-7 and MDA-MB-435 colonies. The number of MCF-7 and MDA-MB-435 colonies was determined after 18 days of culture in DMEM supplemented with 10% FCS containing PE at concentrations of 500 and 1000 μg/mL. Data are presented as mean ± standard deviation of 3 independent experiments, each performed at least in duplicate. ∗indicates significant differences from the control group (∗p < 0.05; ∗∗p < 0.01).
