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. 2017 Jul 12;37(4):BSR20170163. doi: 10.1042/BSR20170163

Figure 10. ODC degradation assay with in vitro translated proteins.

Figure 10

In vitro translated (IVT) proteins were mixed in a 2:1:14 ratio of ODC:OAZ1:ODCrp/Δ1–13ODCrp/AZIN1/lysate, respectively, where only ODC was radiolabeled. The reaction with only ODC and IVT lysate (devoid of translated proteins) shows the basal rate of ODC degradation. Adding OAZ1 (OAZ + IVT lysate) to the reaction increased the rate of degradation. ODCrp (OAZ + ODCrp) and Δ1–13ODCrp (OAZ + Δ1–13ODCrp) did not rescue ODC from the OAZ-mediated degradation, whereas ODC degradation was markedly slowed down in the presence of AZIN1 (OAZ + AZIN1).