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. 2017 Jul 2;2017:7052560. doi: 10.1155/2017/7052560

Figure 2.

Figure 2

Decreased secretion of IL-17A and IFN-γ in both Th17- and Th1-driving conditions by EKC. (a) CD4+ T cells were purified from Balb/c mice and activated with 1 μg/mL of plate-bound anti-CD3/CD28 antibodies in the presence of 25 ng/mL IL-6 and 2.5 ng/mL TGF-β, with or without EKC. Production of IL-17A, IFN-γ, TNF-α, and IL-12p40 was detected by ELISA. (b) Isolated CD4+ T cells were stimulated with plate-bound anti-CD3/CD28 antibodies with or without E. kansui under Th1-driving cytokine conditions (10 ng/mL IL-2, 5 ng/mL IL-12, and 5 μg/mL anti-IL-4) for 3 days. The concentration of cytokines in the supernatant was detected by ELISA. Values represent the mean ± SD. (∗p < 0.05; ∗∗p < 0.01; and ∗∗∗p < 0.001 compared with the DMSO-treated group).