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(A) Western blot assay of TGF-β, BMP-4, and tubulin of HCT116 cells transfected with either control plasmid (CON) or TRIM25 overexpressing plasmid (TRIM25). (B) Western blot assay of p-Smad2, total Smad2, p-Smad4, total Smad4, and tubulin of HT29 cells transfected with either CON or TRIM25.
