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. 2017 Jul 20;8:1338. doi: 10.3389/fmicb.2017.01338

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Copyright © 2017 Sid, Hartmann, Winter and Rautenschlein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of H9N2 (A), pH1N1 (B), and H6N8 (C) in MOC of Pekin duck (Du), chicken (Ch), and turkey (Tu) by FFU (Experiments 1 and 3). MOC were infected with H9N2 (A), pH1N1 (B) or H6N8 (C) (infectious dose of 10⁴FFU/explant). Supernatants were collected at 12, 24, and 48 hpi and were subjected to viral titration with the FFU. Results are presented in FFU/ml log₁₀. Virus-free controls were negative at all investigated time points (n = 5 MOC/group/time point). Different letters indicate differences between groups tested at the same time point post infection p < 0.05, ANOVA, Tukey honestly significant differences (HSD). Error bars indicate standard deviation (SD). (^∗) indicates statistical significance differences between MOC-Tu and MOC-Ch p < 0.05, Wilcoxon Rank-Sum Test. A representative repeat of one experiment is shown.