Table 2.
List of probes and primers for quantitative real-time reverse transcription-PCR in MOC of Pekin duck (MOC-Du).
| RNA target | Primer and probea | Sequenceb (5′-3′) | Accession no.c |
|---|---|---|---|
| IFNα | IFNα F | AGCTTCAGCACCACATCTAC | EF053034 |
| IFNα R | TTCTGGAGGAAGTGTTGGATG | ||
| IFNα P | (FAM)-ACCTTCACCTCAGCACCAACAAGT-(TAMRA) | ||
| IFNλ | IFNλ F | CGGAGGTGCTGAAGTTTAAGA | KJ206897 |
| IFNλ R | GTGTCCACTTCCGATTGAAGA | ||
| IFNλ P | (FAM)-TGAGAACATCACGTCGAAGGACCC-(TAMRA) |
aF, forward primer; R, reverse primer; P, probe.
bFAM, 6-carboxyfluorescein; TAMRA, carboxytetramethylrhodamine.
cGenomic DNA sequence from GenBank.