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. 2017 Jul 20;8:1338. doi: 10.3389/fmicb.2017.01338

Table 2.

List of probes and primers for quantitative real-time reverse transcription-PCR in MOC of Pekin duck (MOC-Du).

RNA target Primer and probea Sequenceb (5′-3′) Accession no.c
IFNα IFNα F AGCTTCAGCACCACATCTAC EF053034
IFNα R TTCTGGAGGAAGTGTTGGATG
IFNα P (FAM)-ACCTTCACCTCAGCACCAACAAGT-(TAMRA)
IFNλ IFNλ F CGGAGGTGCTGAAGTTTAAGA KJ206897
IFNλ R GTGTCCACTTCCGATTGAAGA
IFNλ P (FAM)-TGAGAACATCACGTCGAAGGACCC-(TAMRA)

aF, forward primer; R, reverse primer; P, probe.

bFAM, 6-carboxyfluorescein; TAMRA, carboxytetramethylrhodamine.

cGenomic DNA sequence from GenBank.