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. 2017 Jul 20;2(14):e92570. doi: 10.1172/jci.insight.92570

Figure 2. Relationship between TCR dissociation rates and polyfunctionality of self/tumor– and virus-specific CD8+ T cell clones.

Figure 2

(A) CD107a, IFN-γ, TNF-α, and IL-2 coexpression titration assays of A2/Melan-A26–35– (derived from patient LAU618), A2/NY-ESO-1157–165– (patient LAU155), A2/pp65495–504–, or A2/BMFL1259–267– (healthy donor BCL4) specific clones with slow (n = 10) or fast (n = 10) TCR off-rates. Pie arcs depict the average fraction of cells displaying 0 to 4 functions. (B and C) Individual and (D) average ± SEM polyfunctional (coexpression of CD107a, IFN-γ, TNF-α, and IL-2) titration curves obtained for A2/Melan-A26–35– (derived from patient LAU618), A2/NY-ESO-1157–165– (patient LAU155), A2/pp65495–504–, or A2/BMFL1259–267– (healthy donor BCL4) specific clones with slow (n = 10, plain symbols and solid lines) or fast (n = 10, empty symbols and dotted lines) TCR off-rates. Vertical lines indicate EC50 values. The P values were determined by the extra sum-of-squares F test (α = 0.05). The representative TCR-BV-CDR3 clonotype diversity of each antigenic specificity was LAU618/Melan-A, 80%; LAU155/NY-ESO-1, 45%; BCL4/pp65, 65%; and BCL4/BMFL1, 80%.