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. 2017 Jul 20;2(14):e93735. doi: 10.1172/jci.insight.93735

Figure 1. Accelerated atherosclerosis with hepatic Jak2–deficiency.

Figure 1

ApoE-null mice with hepatic Jak2 deficiency (L-Jak2–/–ApoE–/–) and littermate controls (L-Jak2+/+ApoE–/–) were fed an atherogenic diet containing 0.2% cholesterol for 12 weeks, starting at 6 weeks of age. (A) Representative photographs of en face oil red O (ORO) staining and quantification of atherosclerotic plaque area in descending aortas of L-Jak2–/–ApoE–/– mice (n = 17) and control L-Jak2+/+ApoE–/– mice (n = 13). Scale bar: 1 cm. (B) Representative images of longitudinal sections from the aortic arch of L-Jak2–/–ApoE–/– mice (n = 15) and control L-Jak2+/+ApoE–/– mice (n = 10) stained with H&E and quantification of lesion size at the lesser curvature. B, brachiocephalic artery; C, left common carotid; S, subclavian artery; L, lesser curvature. Arrow indicates necrotic core. Scale bar: 200 μm. (C and D) Representative images of the lesser curvature of longitudinal aortic arch sections from L-Jak2–/–ApoE–/– (n = 10–14) and control L-Jak2+/+ApoE–/– (n = 8–9) mice immunostained with antibody against Mac-3 and α-smooth muscle actin (α-SMA) and quantification of positively stained area expressed as an absolute number or percentage. Scale bars: 100 μm. In each of the panels, each dot in the scatter plot indicates an individual animal. Data represent mean ± SEM. Differences between groups were analyzed for statistical significance by Student unpaired t test. *P < 0.05, **P < 0.01.