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. 2017 Jul 20;2(14):e93735. doi: 10.1172/jci.insight.93735

Figure 2. L-Jak2–/–ApoE–/– mice develop profound hepatic steatosis.

Figure 2

L-Jak2–/–ApoE–/– and L-Jak2+/+ApoE–/– littermate controls were fed an atherogenic diet containing 0.2% cholesterol for 12 weeks, starting at 6 weeks of age. (A) Representative images of H&E and oil red O (ORO) staining of liver sections from L-Jak2–/–ApoE–/– mice (n = 8 and 5) and control L-Jak2+/+ApoE–/– mice (n = 7 and 5). Scale bars: 300 μm (black), 50 μm (gray). (B) Total hepatic triglyceride (TG) content in L-Jak2–/–ApoE–/– mice (n = 6) and control L-Jak2+/+ApoE–/– mice (n = 6). Results are normalized to tissue weight. (C) Liver weight normalized to total body weight in L-Jak2–/–ApoE–/– mice (n = 7) and control L-Jak2+/+ApoE–/– mice (n = 6). (D) Levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in L-Jak2–/–ApoE–/– mice (n = 5–6) and control L-Jak2+/+ApoE–/– mice (n = 3). (E) Quantitative PCR (qPCR) analysis of mRNA expression of genes encoding inflammatory markers (Tnfa, Il6, Ifng) and fibrosis markers (Tgfb, aSma, Procollagen1) in livers from L-Jak2–/–ApoE–/– mice (n = 5–7) and control L-Jak2+/+ApoE–/– mice (n = 4–5). Values are normalized to 18S mRNA levels and presented as fold change over control group. aSma, α-smooth muscle actin. (F) Serum levels of TNFα, IL-6, IFN-γ, and MCP-1 in L-Jak2–/–ApoE–/– mice (n = 5–8) and control L-Jak2+/+ApoE–/– mice (n = 5–7). MCP-1, monocyte chemoattractant protein-1. (G) Total number of white blood cells in red cell–lysed blood from L-Jak2–/–ApoE–/– (n = 5) and control L-Jak2+/+ApoE–/– mice (n = 3). (H and I) Frequency and absolute numbers of Ly6Chi monocytes in red cell–lysed blood from L-Jak2–/–ApoE–/– mice (n = 5) and control L-Jak2+/+ApoE–/– mice (n = 3) using flow cytometry. Ly6Chi monocytes were defined based on CD115+Ly6ChiLy6G– expression. In each of the panels, each dot in the scatter plot indicates an individual animal. Data represent mean ± SEM. Differences between groups were analyzed for statistical significance by Student unpaired t test. *P < 0.05, **P < 0.01.