Figure 3. MED12 regulates calcium-handling genes in the heart.

(A) Heat map of differentially expressed genes in CTL and Med12cKO (cKO) ventricles at P1, analyzed by RNA-Seq with cutoff of P < 0.05 and 1.5 fold change. n = 3. (B) Gene Ontology Analysis and (C) Ingenuity Pathway Analysis of differentially expressed genes in CTL and cKO ventricles. (D) Calcium-handling genes are differentially expressed in CTL and cKO ventricles (RNA-Seq data). (E) qPCR analysis of calcium-handling genes downregulated and (F) upregulated in cKO ventricles. n = 4. All genes in D–F are significantly regulated in Med12cKO ventricles. (G) Protein levels in hearts and CMs from CTL and cKO male mice. Ryr, ryanodine receptor; SERCA2a, sarcoplasmic reticulum Ca²⁺ ATPase 2a; PLN, total phopholamban; CX43, connexin 43. (H) Quantification of Western blots from 6–8 mice. Data are mean ± SEM. *P < 0.05 by 2-tailed Student’s t test.