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. 2017 Jul 20;2(14):e93726. doi: 10.1172/jci.insight.93726

Figure 4. Donor IL-6 production is not required for neuroinflammation during graft-versus-host disease.

Figure 4

(A) BALB/c mice were transplanted with B6 Foxp3EGFP bone marrow (BM) alone (●, n = 6), B6 Foxp3EGFP BM plus B6 Foxp3EGFP spleen cells (■, n = 10), or IL-6–/– Foxp3EGFP BM and IL-6–/– Foxp3EGFP spleen cells (▲, n = 10). The absolute number of donor-derived TCRβ+, CD4+, and CD8+ T cells in the brain 14 days after transplantation is depicted. (B) BALB/c mice were transplanted with B6 BM alone (●, n = 6), BM and spleen cells from Foxp3EGFP mice (■, n = 9), or B6 BM and spleen cells from IL-6–/– Foxp3EGFP animals (▲, n = 9-10). The frequency and absolute number of CD4+ and CD8+ Foxp3+ T cells is shown. (C) IFN-γ, IL-10, and TNF-α mRNA expression in the brains of BALB/c mice transplanted with B6 BM alone (●, n = 6), B6 Foxp3EGFP BM and spleen cells (■, n = 9), or IL-6–/– Foxp3EGFP BM and spleen cells (▲, n = 9) 14 days after transplantation. Statistically significant differences were calculated using the 2-tailed Mann-Whitney U test and the 2-way ANOVA followed by Student’s t test.