Figure 1.

HPLC analysis of serotonin (5-hydroxytryptamine, 5-HT), the serotonin metabolite 5-HIAA, their ratio, and NE in frontal cortex, dorsal striatum (STR), the hippocampal formation (HF), and the dorsal raphe (DR) in C57 (grey) and BTBR (black) mice. (A) BTBR mice have significantly less serotonin in the HF p = 0.004, (B) nonsignificant trends for lower 5-HIAA in several regions, and (C) a reduced ratio of metabolite to transmitter in the striatum (P = 0.006). (D) In addition, BTBR mice have significantly reduced NE content in all the forebrain areas examined (P = 3 × 10−5, 0.04, and 3 × 10−10, respectively). N = 10 per group.