Table 1.
Representative proteins regulating cell growth in CHO K1, CHO S, and CHO/dhfr−.
| Protein | Spectral Counta | ||||
|---|---|---|---|---|---|
|
| |||||
| UniRef IDb | Namec | CHO K1 | CHO S | CHO/dhfr− | Probability |
| G3V6P7 | Myosin, heavy polypeptide 9, non-muscle (myh9) | 52±9.9 | 175±7.1 | 36±11 | 1 |
| Q8BMK4 | Cytoskeleton-associated protein 4 (ckap4) | 19.5±0.7 | 25±7.1 | 7.5±0.7 | 1 |
| B2GV99 | Myl6 protein (myl6) | 15.5±3.5 | 23±4.2 | 10±2.8 | 1 |
| P57780 | Alpha-actinin-4 (actn4) | 60±4.2 | 86±8.5 | 41.5±4.9 | 1 |
| Q58E70 | Tpm3 protein (tpm3) | 17.5±3.5 | 36.5±3.5 | 25.5±3.5 | 1 |
| P09495 | Tropomyosin alpha-4 chain (tpm4) | 23±5.6 | 45±5.7 | 35.5±2.1 | 1 |
| P11440 | Cyclin-dependent kinase 1 (cdk1) | 6.5±2.1 | 13±0 | 4±4 | 1 |
| P68373 | Tubulin alpha-1C chain (tuba1c) | 89.5±0.7 | 122±12.7 | 82±4.2 | 1 |
| Q6P9T8 | Tubulin beta-4B chain (tubb4b) | 93±5.6 | 127±26.8 | 87±4.2 | 1 |
| B7FAU9 | Filamin, alpha (flna) | 134±16.9 | 127.5±4.9 | 0±0 | 1 |
| P20152 | Vimentin (vim) | 216.5±30.4 | 3±0 | 55±0.7 | 1 |
| P45592 | Cofilin-1 (cfl1) | 46.5±2.1 | 37±0 | 20.5±0.7 | 1 |
Note:
a
The average spectral count data with standard deviation that were calculated from triplicated experiments were presented.
b
The annotation was performed using Mouse UniRef100 database. The identified proteins were presented with UniRef100 ID.
c
All raw MS data, search parameters, search database and summarized data with statistic analysis were deposited to a public repository PeptideAtlas (http://www.peptideatlas.org/, accession no. PASS00963).