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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 Jun 2;199(1):159–171. doi: 10.4049/jimmunol.1700214

Figure 3. Conventional CD4, CD8 and Foxp3+ Tregs develop in hemizygous RAG-proficient iNKT TN mice.

Figure 3

(A) Thymocytes from C57BL/6 and all TN lines were stained with anti-CD3, CD1d (PBS57) tetramer, anti-CD4, and anti-CD8. Results shown are gated on non-iNKT cells (CD3+ CD1d-tetramer−). (B) Thymocytes from C57BL/6 and all TN lines were stained with anti-CD3 and CD1d (PBS57) tetramer before being fixed, permeabilized, and stained with anti-Foxp3. Results shown are gated on non-iNKT cells. (C) Quantification of A–B where n=3 mice per group. (D) Lymph node cells from Vβ8.2 and Vβ8.2;RAG2−/− mice were stimulated with the indicated concentrations of α-GalCer. IFNγ was measured by ELISA of 24 hour culture supernatants. Error bars are SD. (E) Each monoclonal TN line was crossed to RAG2−/− background. Peripheral blood of mice of the indicated genotypes was stained with CD1d (PBS57) tetramer and anti-CD3. (F) Quantification of (E) averaged over 5 mice per group.