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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 Jun 2;199(1):159–171. doi: 10.4049/jimmunol.1700214

Figure 6. TCR avidity correlates with PLZF expression during thymic development of monoclonal iNKT cells.

Figure 6

Jα18−/− mice were lethally irradiated, reconstituted with 95% Jα18−/− bone marrow and 5% bone marrow of the indicated TN lines, and analyzed 8 weeks later. (A) Thymocytes from C57BL/6 and all TN lines were stained with anti-CD3 and CD1d (PBS57) tetramer, and analyzed by flow cytometry. Mean fluorescence intensities (MFI) of tetramer staining for gated populations are shown on plots. (B) Thymocytes from C57BL/6 and all TN lines were stained with anti-CD3 and CD1d (PBS57) tetramer before being fixed, permeabilized, and stained with antibodies to PLZF, T-bet, and RORγt. Results shown are gated on CD1d (PBS57) tetramer+ iNKT cells. Tetramer bright = top 20% of iNKT cells; tetramer dim = bottom 20% of iNKT cells as defined by intensity of PE signal. (C) Quantification of mice shown in panel A. (D) Quantification of mice shown in panel B. n=4 mice per group. Error bars are SD.