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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 Jun 2;199(1):159–171. doi: 10.4049/jimmunol.1700214

Figure 7. Limited dilution bone marrow chimeras reveal a minor role for TCR specificity in lineage preference of iNKT cells.

Figure 7

Jα18−/− mice were lethally irradiated, reconstituted with 95% Jα18−/− bone marrow and 5% bone marrow of the indicated TN lines, and analyzed 8 weeks later. n=4 mice per group. (A) Skin draining lymph node cells from all TN mice were stimulated in vitro with PMA/Ionomycin for 4 hours, in the presence of GolgiStop. Cells were stained with anti-CD3 and CD1d (PBS57) tetramer, before being fixed, permeabilized, and stained with antibodies to IFNγ, IL-4, and IL-17. Results shown are gated on CD3+CD1d-tetramer+ cells. (B) Quantification of ICCS data from thymus, spleen, skin-draining lymph nodes and liver, normalized to Vα14 values for the same organ. Error bars are SD.