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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 Jun 2;199(1):159–171. doi: 10.4049/jimmunol.1700214

Figure 8. Tissue of origin plays a dominant role in cytokine profiles of monoclonal iNKT cells.

Figure 8

Jα18−/− mice were lethally irradiated, reconstituted with 95% Jα18−/− bone marrow and 5% bone marrow of the indicated TN lines, and analyzed 8 weeks later. n=4 mice per group. (A) Skin draining lymph node cells from all TN mice were stained with anti-CD3 and CD1d (PBS57) tetramer, before being fixed, permeabilized, and stained with antibodies to PLZF, T-bet, and RORγt. Results shown are gated on CD3+CD1d-tetramer+ cells. (B) Quantification of transcription factor expression as shown in (A). n=4 mice per group. ANOVA was used to determine the relative contribution of TCR versus tissue of origin to explain the variation in transcription factor expression.