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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Mol Biol Rep. 2015 Sep;42(9):1365–1376. doi: 10.1007/s11033-015-3882-x

Figure 5.

Figure 5

Enzyme activity of DNMT1 in HCASMCs cells. Cells were transfected with or without hsa-miR-1264 mimic or its inhibitor and treated with or without TNF-α and IGF-1. DNMT1 activity was quantified in the nuclear extracts. There was significant increase in DNMT1 activity in the cells treated with TNF-α and IGF-1 compared to control (*** p <0.001). However, this increased activity of DNMT1 was completely inhibited by transfecting the cells with hsa-miR-1264 followed by treatment with TNF-α and IGF-1 (**p <0.05). Also, the transfection of the cells with has-miR1264 inhibitor followed by treatment with TNF-α and IGF-1 increased the DNMT1 activity compared to the treatment with TNF-α and IGF-1 (* p <0.01).