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. 2017 May 29;6(8):819–832. doi: 10.1016/j.molmet.2017.05.011

Figure 3.

Figure 3

Precursor availability and skeletal muscle NAD+biosynthesis. (A) Primary myotube NAD+ levels following 24 h 0.5 mM NAD+ precursor supplementation (n = 4) determined using an NAD+ cycling assay. (B) Primary myotube NAD+ content following 24 h 0.5 mM NAD precursor supplementation with or without FK866 inhibition of NAMPT (* = significant from DMSO control and # = significant from FK866 treated control) (n = 4) determined using the NAD+ cycling assay. (C) Basal and maximal mitochondrial respiration following 24 h NR supplementation with or without FK866 inhibition (n = 3). (D) Transcriptional regulation of NAD+ biosynthesis genes, Nmrk1, Nmrk2, and Nampt following 24 h NR supplementation with or without FK866 inhibition of NAMPT (n = 3).