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. Author manuscript; available in PMC: 2018 Jun 29.
Published in final edited form as: Chem Commun (Camb). 2017 Jun 29;53(53):7349–7352. doi: 10.1039/c7cc03455e

Fig. 1.

Fig. 1

Schematics of protein synthesis in liposomes and gene circuits. (A) dsDNA is transcribed into mRNA, which is then translated to a biosensing fluorescent reporter protein (G-GECO) and a mechanosensitive membrane-active protein (MscL). (B) Gene circuits used in this work. (i) The E. coli housekeeping transcription factor sigma 70 activates the promoter P70a. (ii) The sigma 28 cascade requires the expression of sigma 28 protein, expressed through the P70a promoter. The sigma 28 transcription factor activates the corresponding promoter P28a.