Figure 2.

Early onset obesity in ob/ob mice downregulates adipose tissue DNL, but not AKT activation. (A): 7 week-old WT or ob/ob mice were fasted overnight and subjected to a 2 h hyperinsulinemic-euglycemic clamp with [¹⁴C]-glucose to assess glucose conversion into lipids. Adipose tissues were then harvested and lipid extracted to determine glucose incorporation into TG or fatty acids. (B): Insulin-stimulated [¹⁴C]-glucose conversion into TG and into TG-fatty acids were measured ex vivo in iWAT derived from 7 or 11 week old WT or ob/ob mice. (C): Representative protein immunoblot of AKT phosphorylation in iWAT in response to a bolus insulin injection (1 U/kg) as described in Materials and Methods. Bottom panels depict densitometry analysis of the data from the experiment described for top panel (N = 6 or 7). Graphs show the mean −/+ SEM. N = 5 to 7 per group, compared with WT controls, by Student's t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.