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. 2017 May 31;6(8):781–796. doi: 10.1016/j.molmet.2017.05.012

Figure 5.

Figure 5

Inducible deletion of adipocyte FASN in mature mice enhances browning of iWAT. (A): qRT-PCR was performed for Ucp1, Cidea, Pgc1a, and Pdk4 mRNA for quantifications in iWAT from indicated controls and iAdFASNKO (Cre+, TAM-treated) mice. N = 5–6 per group. (B): Inducible deletion of adipocyte FASN upregulates UCP1 protein in iWAT. iWAT lysates from controls and TAM-treated mice were immunoblotted for FASN, UCP1 or GAPDH as indicated. (C): Immunohistochemistry for UCP1 of iWAT from control or iAdFASNKO mice reveals UCP1 upregulation in the latter. Multilocular adipocytes are also detected in iAdFASNKO mice, but not control mice. Depicted are representative images of 5–7 mice per group. (D–G): qPCR analysis to quantify mRNA expression levels of Fasn, Ucp1, Cidea and Pdk4 in anterior subcutaneous (asWAT), epididymal (eWAT), retroperitoneal (rpWAT) and mesenteric (mWAT) adipose tissues from control or iAdFASN KO mice as indicated. N = 8 per group. *P < 0.05; **P < 0.01; ****P < 0.0001.