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. 2017 Jun 7;6(8):909–921. doi: 10.1016/j.molmet.2017.06.002

Figure 1.

Figure 1

PPG neurons receive close appositions from 5-HT immunoreactive axons. Two-color immunoperoxidase labeling for YFP-immunoreactivity in YFP–PPG neurons (brown) and 5-HT-immunoreactivity in serotonergic neurons (black) in transverse sections through the dorsal vagal complex (DVC) of YFP–PPG mice. (A) Montage of low magnification micrographs showing YFP-immunoreactive PPG cell bodies (brown) and 5-HT-immunoreactive axons (black) in the DVC. The cell body indicated by the arrow is shown at higher magnification in B. Scale bar = 100 μm. (B, C, D) Brown YFP-immunoreactive cells bodies and dendrites in the NTS receive close appositions from black 5-HT-immunoreactive axons. Scale bars = 10 μm. (E) A YFP-immunoreactive cell body in the IRT receives close appositions from 5-HT-immunoreactive axons. Scale bar = 20 μm. AP: area postrema; cc: central canal; DMV: dorsal motor nucleus of the vagus; HGN: hypoglossal nucleus; NTS: nucleus tractus solitarius.