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. 2017 Jun 7;6(8):909–921. doi: 10.1016/j.molmet.2017.06.002

Figure 5.

Figure 5

PPG neurons are activated by 5-HT via 5-HT2 but not 5-HT3 receptors and respond to 5-HT2C receptor activation with an increase in dendritic Ca2+. (A) Left panel: Traces showing the responses to 20 μM 5-HT or 10 μM PBG (n = 7 dendrites), with individual fibers shown in gray and the average trace in red. Right panel: Median AUC during the response to 20 μM 5-HT (n = 37 dendrites), 1 μM PBG (n = 32 dendrites) or 10 μM PBG (n = 25 dendrites) (3 mice). Kruskal–Wallis rendered a Chi-Square value of 45.9 (p < 0.0001). Post-hoc comparisons revealed no statistically significant difference between 1 μM and 10 μM PBG (p > 0.999) but showed that the responses to 20 μM 5-HT were significantly different to both 1 μM and 10 μM PBG (p < 0.0001). (B) Left panel: Traces showing the response to 20 μM 5-HT in the absence and presence of 5 μM granisetron (granisetr.; n = 6 dendrites). Individual fibers are shown in gray and the average trace in red. Right panel: Median AUC for the response to 20 μM 5-HT before (white box) and during (gray box) 5 μM granisetron (n = 32 dendrites (3 mice)). Wilcoxon test: p = 0.12. (C) Left panel: Representative traces showing the response to 20 μM 5-HT in the absence and presence of 1 μM ketanserin (n = 6 dendrites). Right panel: Median AUC for the response to 20 μM 5-HT in the absence (white box) or presence (gray box) of 1 μM ketanserin (n = 17 dendrites (3 mice)). Wilcoxon test: p < 0.0001. (D) Left panel: Representative traces showing the response to 5 μM WAY161,503, a 5-HT2C receptor agonist (n = 9 dendrites). Right panel: Median AUC for the response to 1 min 5 μM WAY161,503 (n = 32 dendrites (5 mice)). n.s.: not significant.