Skip to main content
. 2017 Jun 20;6(8):897–908. doi: 10.1016/j.molmet.2017.06.008

Figure 1.

Figure 1

High carbohydrate, high fat diets, but not low-carbohydrate, high-fat diets, increase hypothalamic microglial reactivity and neuronal CML content. (A) Daily calorie intake of chow, HCHF1, HCHF2, LCHF1, and LCHF2 diet groups (n = 13 for each group, F(1,60) = 80.26, P < 0.0001). (B & C) Independent of daily calorie intake, the HCHF1 and HCHF2 groups had the most profound impact on BW gain (n = 13 for each group). (D) Fat mass proportion of total BW of each diet group (n = 13 for each group). (E) Quantification of the number of iba1-ir microglia in a frame of 0.2 mm × 0.2 mm in the ARC (illustrated in I) from each diet group (n = 6 for each group). (F) Quantification of the cell coverage of iba1-ir microglia. (G) Quantification of the area covered by GFAP-ir astrocytes in a frame of 0.2 mm × 0.2 mm in the ARC (illustrated in I) from each diet group (n = 6 for each group). (H) Quantification of the integrative intensity of CML-ir in a frame of 0.2 mm × 0.2 mm in the ARC (illustrated in J) from each diet group (n = 6 for each group). (I) Illustration of iba1-ir microglia in the ARC of each diet group; dark arrow pointed cells are magnified in the left upper corner. (J) Illustration of GFAP-ir astrocytes in the ARC of each diet group. (K) Illustration of CML-ir cells in the ARC of each diet group. III: third cerebral ventricle; Scale bar: 50 μm in I and K, and 100 μm in J. *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as means ± s.e.m. P values for 5-diet group-effects were analyzed by One-way ANOVA, followed by Student's t test for post-hoc analysis.