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. 2017 Jun 20;6(8):897–908. doi: 10.1016/j.molmet.2017.06.008

Figure 4.

Figure 4

Deletions of RAGE or ALCAM genes improve metabolic symptoms induced by a HCHF diet and exert diverse impacts on microglia, pericytes, and vasculature in the arcuate nucleus. (A & B) Daily caloric intake (in wk10) and weekly BW gain of chow or HCHF diet-fed WT versus RAGE−/− mice (n = 5–8 per group); For weekly BW gain, in all time points, WT and RAGE−/− mice have less BW gain on chow diet than on HCHF diet (P < 0.0001); from wk14 to wk16, BW gain on HCHF of RAGE−/− mice is significantly less than WT mice. (C & D) Daily caloric intake (in wk10) and weekly BW gain of chow or HCHF diet fed WT mice versus ALCAM−/− mice (n = 5–8 per group). For weekly BW gain, from wk2 on, WT and ALCAM−/− mice have less BW gain in chow than in HCHF; from wk12, wk14 to wk16, BW-gain on HCHF of ALCAM−/− mice is significantly less than WT mice. (E & F) Quantification of the number of iba1-ir microglia and the PDGFRβ-ir pericytes in the ARC in chow or HCHF diet fed WT mice versus RAGE−/− mice (n = 5–9 per group). (G & H) Quantification of vessel length and vascular density in the ARC from chow or HCHF diet fed WT mice versus RAGE−/− mice (n = 5–7 per group). (I & J) Quantification of the number of iba1-ir microglia and the PDGFRβ-ir pericytes in the ARC in chow or HCHF diet fed WT mice versus ALCAM−/− mice (n = 5–6 per group). (K & L) Quantification of vessel length and vascular density in the ARC from chow or HCHF diet fed WT mice versus ALCAM−/− mice (n = 6 per group). Data are presented as means ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisks in B and D indicate significance between WT and RAGE−/− or ALCAM−/− mice on HCHF diet. Two-way ANOVA followed by Bonferroni multiple comparisons for post-hoc analysis was performed to detect significant interaction between genotype and diet on each parameter.