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. 2017 May 31;6(8):833–844. doi: 10.1016/j.molmet.2017.05.016

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IL-1Ra release from Ad-prohIAPP-siRNA transduced and non-transduced human islets cultured with or without neutralizing IL-1β antibody. (A, B) IL-1Ra release from human islets transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA following 3 or 7 days culture (11.1 mmol/L glucose) in the presence (+nIL1β) or absence (-nIL1β) of neutralizing IL-1β antibody. (C) Comparison between islet IL-1Ra release following 3 and 7 days culture in each condition. Data are expressed as mean ± SEM of four independent studies (4 human islet preparations) performed in duplicate. *vs corresponding day 3 group (p < 0.05, two-way ANOVA).