Fig. 2.

Clinical signs and viral replication in vaccinated animals. Animals were vaccinated twice at Day 0 and Day 21 (boost). (A) Clinical signs were scored based on body temperature, breathing rhythm, cardiac pulses, etc. Four animals were vaccinated with ECRA.A4 (group A; top panel), four with a cocktail of ECRA.A1/4/6/8 (group B; lower panel) and two animals were used as control (C1 & C2). (B) AHSV genomic RNA in serum was determined by RT-PCR and expressed as Ct values. Virus load is represented by a colour gradient from green (ND: not detected) to red (Ct less than 25). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)