Table 1B.
Serum neutralization activity in ponies after virulent virus challenge. Neutralizing antibody titers of vaccinated animal sera were determined by SN assay at day 8 and day 24 (44 and 60 days post challenge) against serotypes AHSV 1, 4, 5, 6 and 8. Titers were expressed as the reciprocal of the highest dilution of sera allowing complete neutralization. Both C1 and C2 ponies were euthanized respectively at 11 days and 10 days’ post challenge due to severe AHS symptoms. Not determined (nd) and not detected (–) are indicated.
| Group | Animal ID | Neutralizing antibody titers |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 days after challenge |
24 days after challenge |
||||||||||
| AHSV 1 | AHSV 4 | AHSV 5 | AHSV 6 | AHSV 8 | AHSV 1 | AHSV 4 | AHSV 5 | AHSV 6 | AHSV 8 | ||
| A ECRA.A4 |
A1 | nd | 64 | nd | nd | nd | nd | 32 | nd | nd | nd |
| A2 | nd | 32 | nd | nd | nd | nd | 16 | nd | nd | nd | |
| A3 | nd | ≥256 | nd | nd | nd | nd | 64 | nd | nd | nd | |
| A4 | nd | 64 | nd | nd | nd | nd | ≥256 | nd | nd | nd | |
| B ECRA .A1/4/6/8 |
B1 | 16 | 32 | – | 4 | 8 | 8 | 16 | – | 4 | 8 |
| B2 | – | 64 | – | – | 8 | 4 | 32 | – | – | 4 | |
| B3 | 4 | ≥256 | 4 | 16 | 32 | 4 | 128 | – | 16 | 32 | |
| B4 | – | ≥256 | 4 | – | 8 | – | 32 | – | – | – | |
| C Control |
C1 | – | 4 | – | – | – | nd | nd | nd | nd | nd |
| C2 | – | – | – | – | – | nd | nd | nd | nd | nd | |