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. 2017 Jun 22;6(8):882–896. doi: 10.1016/j.molmet.2017.06.011

Figure 2.

Figure 2

Figure 2

Assessment of Cre-activity in Ghsr-IRES-Cre mice. Inset shows the schematic of YFP reporter expression in GHSR-expressing cells in mice derived from the cross of Ghsr-IRES-Cre mice with Rosa26-YFP reporter mice. Cre-mediated excision of the loxP-flanked STOP cassette allows for YFP reporter expression in cells with Cre activity. A–Z. Series of low-power bright-field representative photomicrographs summarizing YFP-immunoreactive cell bodies in coronal brain sections of Ghsr-IRES-Cre X ROSA26-YFP reporter mice. Immunoreactivity is demonstrated by the dark brown DAB stain. Scale bar in panel Z = 500 μm (applies to A–Z). Approximate distance of the coronal section from bregma (B) is indicated for panels A–Y. 3V – third ventricle, 4V – fourth ventricle, Aq – Aqueduct of Sylvius, BSTS – supracapsular part and BSTMV – medial division, ventral part of the BNST, D3V – Dorsal 3rd ventricle, GM – gray matter of the spinal cord, GrDG – Granular layer of Dentate Gyrus, LV – lateral ventricle, Py – Pyramidal cells. See Table 1 for explanation of abbreviations of the brain regions.