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. 2017 Feb 20;14(6):557–567. doi: 10.1038/cmi.2016.65

Figure 5.

Figure 5

Gαq-KO impairs IL-6 production both in vivo and in vitro. (a) qPCR analysis of il6, il23, il1β, tnfα, and tgfβ mRNA expression in splenocytes from WT or Gαq-KO EAE mice. (b) Serum was collected from WT mice and Gαq-KO EAE mice on day 10 PI, and the IL-6 level was measured. (c-e) BMDCs were induced and further stimulated with LPS (1 μg/ml) for 24 h, and the IL-6 (c), IL-23 (d), and TNF-α (e) in the culture supernatant were measured. (f, g) Th17 differentiation was monitored with FACS analysis in the in vitro DC–T cell co-culture system, the representative FACS (f) and statistics data (g) are shown. The data are expressed as the mean±s.e.m. (n=3), *P<0.05, **P<0.01, ***P<0.001 versus the WT group.